April32012
1. HIV has surface proteins called gp120 that attach to cells with CD4 receptors on their surfaces. CD4 is found on helper T-Cells, and on macrophages of the immune system.
2. The HIV virus binds with the cell, and the soon to be host cell incorporates the virus’s membrane into its own and the viral core enters the host cell. Upon entrance, the virus loses its membrane—allowing it to introduce its RNA into the cytoplasm.
3. Viral enzymes convert the viral RNA into DNA, and the viral enzyme reverse transcriptase copies the RNA into complementary DNA (cDNA). Reverse transcriptase has a high error rate, and frequently mutates the copied DNA—allowing the virus to evolve quickly.
4. The viral RNA is destroyed by another viral enzyme called ribonuclease H, and then reverse transcriptase synthesizes a second DNA strand using the first as a template.
5. The double stranded viral DNA enters the nucleus with the help of viral proteins, and integrase enzymes splice the viral DNA into the cell’s chromosomal DNA. This strand is known as provirus.
6. The synthesis of the viral genome begins with the transcription of this proviral DNA into RNA, which contains the code to produce capsid proteins (and others) needed for viral assembly.
7. In this spliced form, the RNA codes also for the envelope proteins and other auxiliary proteins. These are produced in the rough ER, then moved through the golgi before arriving at the cell surface.
8. Full length viral RNA molecules, along with all other viral components, assemble at the membrane and bud off the cell. It’s not clear how HIV stops the immune response, nor how it causes the destruction of T cells—it ultimately results in the loss of all functional T helper cells in the immune system.
March222012
Antibodies - Your body’s Kamikaze
Opsonization is the process of ingestion and destruction of a pathogen by a phagocite. It starts with the antibodies recognizing the antigen on the bacteria and attaching to the epitope of the antigen. Once the anitbody attaches to the antigen’s epitope, the Fc region of the antibody changes structure. The changing of the structure of the Fc region signals the Fc receptor on the phagocite to attach to the Fc region, and engulf the bacteria—along with the antibody—and destroy it all.
The process of opsonization for viruses is slightly different, as the virus infects cells instead of being an actual body as is the bacterium. The host cell indicates that it is infected by the virus by putting out flag antigens, which signal to antibodies to attach to the antigen’s epitope. When the antibody attaches to the epitope, the structure of the Fc region changes, signaling to the Fc receptor that it needs to destroy the host cell, virus, and antibodies.